Reporter
Part:BBa_K5317002:Design
Designed by: Jan Gelhoet Group: iGEM24_Hannover (2024-09-06)
miRFP670
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 763
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 763
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 419
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 763
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 763
Illegal NgoMIV site found at 82
Illegal NgoMIV site found at 370
Illegal NgoMIV site found at 405 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The miRFP670 was imported from start to stop codon.
Source
The miRFP670 was amplified from the FUCCIplex plasmid.